Galactosylated CII peptide (CII259-273-GalHyl264) – 100µg


Product number #10550

For studies on T-Cell Responses Associated with Autoimmune Arthritis


IMG_20250306_165659
Size and Price

100 µg

Please contact info@vacara.se to request a quote.


Description


The Galactosylated CII peptide (CII259-273-GalHyl264) contains the 259 to 273 sequence of rat CII with with a β-D-galactopyranosyl residue on L-hydroxylysine at position 264 (GalHyK264). It is the dominant T-cell epitope in collagen induced arthritis (CIA) in mice1. The Galactosylated CII peptide (CII259-273-GalHyl264) activates autoimmune T-cells when presented by the MHC II Aq protein2. The Galactosylated CII peptide (CII259-273-GalHyl264) can be used as an in vitro stimulus in antigen-specific T-cell recall assays for quantitation of T cell activity 1,2,3,4,5 by determining the selected readout by ELISA, ELISpot, flow cytometry or RNAseq, e.g. 


Sequence

H2N-Gly-Ile-Ala-Gly-Phe-Lys{(R)-δ-hydroxy-(β-D-galactopyranoside}-Gly-Glu-Gln-Gly-Pro-Lys-Gly-Glu-Thr-COOH

Peptide backbone sequence origin

Collagen alpha-1(II) chain, COL2A1, type II collagen, CII; UniProt: P05539. 

Size

100 µg; á 20µl

Supplied as

5 µg/µl in PBS 

Molecular weight

1653.76 g/mol

Storage

It is recommended to store the peptide in aliquots at -20°C, or preferably at -80°C, unless stability data would suggest otherwise. If the peptide is stored less than 48 hours before use, the peptide may be stored at 5°C (or room temperature). Avoid repeated thawing and freezing procedures.

Recommended dilution

It is recommended that the user determines the optimal dilution for their application. For in vitro T cell stimulation, a concentration of 10 μg/ml 5 and a concentration of 20 µg/ml for IL-17 ELISpot assays4 has been used.


          

For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.

 

References

  1. Michaëlsson E, Andersson M, Engström A, Holmdahl R. Identification of an immunodominant type-II collagen peptide recognized by T cells in H-2q mice: self tolerance at the level of determinant selection. Eur J Immunol. 1992 Jul;22(7):1819-25. PMID: 1378019
  2. Andersson IE, Andersson CD, Batsalova T, Dzhambazov B, Holmdahl R, Kihlberg J, Linusson A. Design of glycopeptides used to investigate class II MHC binding and T-cell responses associated with autoimmune arthritis. PLoS One. 2011 Mar 15;6(3):e17881.  PMID: 21423632.
  3. Lindgren C, Andersson IE, Berg L, Dobritzsch D, Ge C, Haag S, Uciechowska U, Holmdahl R, Kihlberg J, Linusson A. Hydroxyethylene isosteres introduced in type II collagen fragments substantially alter the structure and dynamics of class II MHC A(q)/glycopeptide complexes. Org Biomol Chem. 2015 Jun 14;13(22):6203-16.. PMID: 25960177.
  4. Klocke K, Sakaguchi S, Holmdahl R, Wing K. Induction of autoimmune disease by deletion of CTLA-4 in mice in adulthood. Proc Natl Acad Sci U S A. 2016 Apr 26;113(17):E2383-92. PMID: 27071130.
  5. Merky P, Batsalova T, Bockermann R, Dzhambazov B, Sehnert B, Burkhardt H, Bäcklund J. Visualization and phenotyping of proinflammatory antigen-specific T cells during collagen-induced arthritis in a mouse with a fixed collagen type II-specific transgenic T-cell receptor β-chain. Arthritis Res Ther. 2010;12(4):R155. PMID: 20682070