Affinity purified chimeric human-mouse monoclonal anti- collagen type II (COL2A1) antibody clone CB20 in PBS
| 100 µg / 0.1 mL | 249 € |
| 1 mg / 1 mL | 1,200 € |
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Affinity purified chimeric human-mouse monoclonal anti- collagen type II (COL2A1) antibody clone CB20 in PBS, produced in HEK293 cells. The CB20 antibody1,2 recognizes the C1 epitope on triple helical collagen II. The CB20 antibody tolerates the citrullination of Arg360, but citrullination of Arg365 abolishes binding3.
Description
The CB20 antibody may be used for in vivo binding studies to locate the C1-epitope in tissues or for immunohistochemistry (IHC) with ex vivo cryofixed tissues, e.g. on collagen in the joints4. The CB20 antibody has been used to specifically block C1-epitope specific antibodies in serum5.
The CB20 antibody, provided as human IgG1, is ideal for staining tissue sections from other species such as experimental mice and rats, where anti-human IgG specific reagents may be used for detection without cross reactivity to the IgGs in the sample.
Target with alternative names
Collagen alpha-1(II) chain, COL2A1, type II collagen, CII; UniProt: P28481.
Epitope
Mouse collagen II triple helical C1 epitope (position 359-369), with sequence ARGLTGRPGDA6, with binding focus on the latter Arginine7.
Species reactivity
Mouse, rat, human, chicken
Specificity
The CB20 antibody recognizes the conformational C1 epitope on the triple-helical structure of the native CII molecule.
Isotype
Human IgG1, κ
Concentration
1 mg/ml
Sizes available
100µg and 1mg, biotinylated version available (#11201)
Supplied in
PBS
Storage
Centrifuge briefly prior to opening vial. Store at +4 °C short term (1-2 weeks). Aliquot and store at ‑20 °C long term. Avoid repeated freeze/thaw cycles.
It is recommended that the user determines the optimal dilution for their application. The typical starting working dilutions are: Functional assays (cell culture) 1:200-1:500, IHC 1:1000.
For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.
References
2. Karlsson R, Mo JA, Holmdahl R. Binding of autoreactive mouse anti-type II collagen antibodies derived from the primary and secondary immune response investigated with the biosensor technique. J Imm Meth. 1995: 8551039
3. Haag S, Schneider N, Mason DE, Tuncel J, Andersson IE, Peters EC, Burkhardt H, Holmdahl R. Identification of new citrulline-specific autoantibodies, which bind to human arthritic cartilage, by mass spectrometric analysis of citrullinated type II collagen. Arthritis Rheumatol. 2014 Jun;66(6):1440-9. PMID: 24470447.
4. Aoun M, et al, . Antigen-presenting autoreactive B cells activate regulatory T cells and suppress autoimmune arthritis in mice. J Exp Med. 2023 Nov 6;220(11):e20230101. Erratum in: J Exp Med. 2023 Nov 6;220(11):e2023010109212023c. PMID: 37695523.
5. Li Q, Li Y, Liang B, Xu R, Xu B, Lönnblom E, Feng H, Bai J, Stawikowska R, Ge C, Lu A, Fields GB, Xiao L, Holmdahl R. Rheumatoid arthritis sera antibodies to citrullinated collagen type II bind to joint cartilage. Arthritis Res Ther. 2022 Nov 22;24(1):257. PMID: 36419093.
6. Uysal H, Bockermann R, Nandakumar KS, Sehnert B, Bajtner E, Engström A, Serre G, Burkhardt H, Thunnissen MM, Holmdahl R. Structure and pathogenicity of antibodies specific for citrullinated collagen type II in experimental arthritis. J Exp Med. 2009 Feb 16;206(2):449-62. PMID: 19204106.
7. Knapinska AM, Tokmina-Roszyk D, Haag S, Lauer-Fields JK, Singh C, Stawikowska R, He Y, Askling J, Holmdahl R, Fields GB. Matrix metalloproteinase 13 (MMP-13) processing of type II collagen is altered by antibodies and citrullination found in the early stages of rheumatoid arthritis. Matrix Biol. 2025 Dec;142:46-57.. PMID: 41197961.
