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Anti-citrullinated COL2A1 Antibody (ACC1) - Human IgG1

Anti-citrullinated COL2A1 Antibody (ACC1) - hIgG1

Product number #11130 


Affinity purified mouse monoclonal anti- citrullinated collagen type II antibody clone ACC1 in PBS, sterile filtered (0.2 µm)


Size and Prices

100 µg / 0.1 mL328 €
1 mg / 1 mL1,548 €


For bulk orders, please contact info@vacara.se to request a quote.

Price is excluding shipping cost. The shipping cost will be added on.

 

Product details

The Anti-citrullinated COL2A1 Antibody clone ACC1 binds to the triple helical C1 epitope on collagen II when citrullinated. Binding to collagen II epitopes was determined in bead-based flow immunoassay as median fluorescence intensity (MFI).


Description

Recombinant, affinity purified, chimeric human-mouse monoclonal anti collagen II (COL2A1) antibody clone ACC1 in PBS, sterile filtered (0.2 µm). The antibody binds to the citrullinated triple helical collagen type II (CII) epitope (position 359-369; ARGLTGRPGDA)1, the C1 epitope. The ACC1 antibody recognizes the second citrulline at position 365 in mouse CII1. The antibody binds to flexible triple-helical CII determinants as well as many citrullinated CII peptides and was found to cross-react with several non-citrullinated epitopes on native CII2. The antigen or epitope-binding specificity of this antibody is identical to its mouse IgG2a variant (#10130)1,2.


Target with alternative names

COL2A1, Collagen alpha-1(II) chain, CII, Col II, citrullinated CII; citrullinated Col II; citrullinated proteins. UniProt: P28481.


Immunogen

Bovine Collagen type II 1


Species reactivity:

Mouse, human


Isotype

Human IgG1, κ


Specificity

ACC1 antibody binds the citrullinated C1 epitope. The ACC1 antibody cross-reacts with triple-helical epitopes on CII, but not with other collagens or the proteins tested in Ge et al2, except for a few citrullinated peptides.2


Concentration

1 mg/ml


Supplied in

PBS


Storage

Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -‑20°C long term. Avoid repeated freeze/thaw cycles.


Recommended dilution

It is recommended the user determines the optimal dilution for their application. The typical starting working dilutions are: IHC 1:100, ELISA 1:200 - 1:500.



For Research Use Only. Not for use in diagnostic procedures.
Not for resale without express authorization.

References

  1. Uysal H, Bockermann R, Nandakumar KS, Sehnert B, Bajtner E, Engström A, Serre G, Burkhardt H, Thunnissen MM, Holmdahl R. Structure and pathogenicity of antibodies specific for citrullinated collagen type II in experimental arthritis. J Exp Med. 2009 Feb 16;206(2):449-62. PMID: 19204106.
  2. Ge C, Tong D, Liang B, Lönnblom E, Schneider N, Hagert C, Viljanen J, Ayoglu B, Stawikowska R, Nilsson P, Fields GB, Skogh T, Kastbom A, Kihlberg J, Burkhardt H, Dobritzsch D, Holmdahl R. Anti-citrullinated protein antibodies cause arthritis by cross-reactivity to joint cartilage. JCI Insight. 2017 Jul 6;2(13):e93688. PMID: 28679953.

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FAQs

Q1: What epitopes does ACC1 bind?

Q2: Can ACC1 induce experimental arthritis?

    The mouse IgG2a ACC1 has been extensively characterized in animal models, one typical utilization is for collagen antibody induced arthritis (CAIA). However, ACC1 alone cannot induce CAIA, it is usually combined with other monoclonal antibodies (e.g. M2139) as a cocktail to enhance the arthritogenic potency. 

    https://www.nature.com/articles/s41467-023-36257-x

Q3: Which assays can ACC1 be used for?

    Taking the advantage of the well characterized epitope specificity, the antibody is highly flexible for adaptation in different purposes. The most common assay is ELISA, where the antibody could be used for capturing certain citrullinated or native COL2 epitopes. In immunohistochemistry (IHC) analysis, ACC1 binds strongly to naive or arthritic mouse joint cartilage. It also binds to psoriatic skin and esophagus tissue, ressembling the nature of typical ACPA.

    https://link.springer.com/article/10.1186/s13075-022-02945-0

Q4: Which secondary antibody should I choose for ACC1?

  • For the mouse IgG2a, we have tested the antibody in multiple assays and can confirm that conventional anti-mouse IgG products are ideal as a secondary antibody. Optionally, anti-mouse kappa secondary antibody also performs similarly well. Similarly, for the human IgG1 variant, please choose the secondary antibody accordingly.

Q5: Do you provide labeled/tagged version of this antibody?

  • We have biotinylated version for most of our monoclonal antibodies, please contact us for details.